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HA-tagged IL13Rα2 expression from the individual clones of stable transfectants of the J1.1 cell line. The individual clones were amplified as detailed in Materials and Methods. The cells were harvested from 25-cm2 flasks, solubilized, and subjected to electrophoresis in 12% denaturing polyacrylamide gels, electrically transferred onto a nitrocellulose sheet, and reacted with a polyclonal antibody to HA tag.
