Table 1.

Summary of relative enzymatic activities for circularly permuted Bla proteins

Enzyme name	New terminal ends, C′/N′	Relative rate, % of WT
WT Bla	—	100
CP-7	254/255	<3.5
CP-8	198/199	16
CP-9	187/188	<1.2
CP-11	215/216	<0.1
CP-12	217/218	<2.0
CP-13	268/269	4.4
CP-14	269/270	9.0

N′ and C′ denote the new N-terminal and C-terminal ends, respectively, of each circularly permuted enzyme and are numbered according to the accepted convention for class A Bla proteins (15).