Fig 5.

Number of start/stop bouts of the two sets of appropriate Control flies (Ace, males treated with the acetone vehicle only; Flu-Ace, males treated with fluvastatin + acetone) compared with males who received either only methoprene (Met) or both fluvastatin and methoprene (Flu-Met). The number of flies observed is shown in each box. Values given are means ± SEM. *, P < 0.05; **, P < 0.01. The statistical difference between the fluvastatin-treated males that received methoprene compared with the males that received only fluvastatin indicates that methoprene is sufficient to reverse the effect (JH deficiency) caused by fluvastatin. The relatively lower number of start/stop bouts observed in this experiment, compared with Fig. 4, could be attributable to deleterious secondary side effects caused by the acetone vehicle. Nevertheless, the reversal effect is clearly apparent.