Fig 5.

HGF-dependent MAP-kinase phosphorylation in Met^{grb2/grb2neo−}. (A and C) Representative Western blots of extracts of cerebellar granule cell cultures from P6 WT and mutant mice, stimulated with HGF (20 ng/ml) (A) or BDNF (100 ng/ml) (C) for the indicated times. Blots were probed with antiphospho-MAP kinase, stripped, and reprobed with an antibody against β-actin for normalization. (B and D) Densitometric analysis of the Western blots. Scanned films were analyzed with the IMAGE MASTER TOTAL LAB V100 program (Amersham Pharmacia) and plotted with Microsoft EXCEL. Values were obtained from eight different experiments and are presented as mean ± SEM.