Table 3.

Effect of DNA ligase on the fidelity of amplification.^a,b

Protocol		Affymetrix	Affymetrix w/o ligase
Number of amplifications		6	5
Correlation coefficient	Virtual	0.84	0.86
	Average	0.79 ± 0.04	0.82 ± 0.04
Yield of aRNA (μg)		24.1 ± 4.7	19.2 ± 5.9
Fold of amplification		803 ± 157	640 ± 197

^aData were obtained from comparing expression level of 13,783 clones using the described selection criteria. ^bInput BC2 total RNA is 3 μg.