Table 4.

Effect of column cleanup on the fidelity and yield of amplification.^a,b

Protocol		G-50 cleanup	w/o G-50 cleanup
Number of amplifications		7	5
Correlation coefficient	Virtual	0.83	0.85
	Average	0.79 ± 0.03	0.81 ± 0.02
Yield of aRNA (μg)		11.9 ± 2.8	15.9 ± 2.7
Fold of amplification		397 ± 93	530 ± 90

^aData were obtained from comparing expression level of 12,305 clones using the described selection criteria. ^bInput BC2 total RNA is 3 μg and amplification was done without TS primer.