Table 9.

The effect of the amount of template BC2 total RNA on the fidelity, reproducibility and yield of amplification.^a

Input total RNA		3 μg	1 μg	300 ng	100 ng	30 ng	10 ng
T7 primer used (μg)		0.5	0.2	0.1	0.1	0.1	0.1
Average correlation coefficient	Amplified vs. gold standard (fidelity)	0.80 ± 0.04 (n = 4)	0.81 ± 0.05 (n = 3)	0.84 ± 0.05 (n = 2)	ND	ND	ND
	Amplified vs. amplified (reproducibility)	0.92 ± 0.04 (n = 4)	0.88 ± 0.04 (n = 3)	0.90 (n = 2)	ND	ND	ND
Yield (μg)		15.2 ± 5.6 (n = 4)	7.4 ± 4.6 (n = 3)	3.1 ± 1.6 (n = 2)	0.60 ± 0.3 (n = 2)	0.33 ± 0.2 (n = 3)	0.11 ± 0.1 (n = 3)
Fold of amplification		507 ± 186	740 ± 460	1033 ± 530	600 ± 300	1100 ± 667	1100 ± 1000

^aData were obtained from comparing expression levels of 13,164 clones using the described selection criteria. ND = not determined due to insufficient yield of aRNA for microarray hybridization after single round of amplification.