Table 3.
Real-time PCR estimates of copy number for TSWV-N and nptII transgenes and possible rearrangements. For each line and each gene the estimate of copy number was derived from values in Table 2, as the range of integers that are included in the 95% confidence interval around the ratio rline/r1. The column on the right indicates evidence for rearrangements in the T-DNA.
| Line | TSWV-N | nptII | rearranged |
| 111–6 | 1 | 1 | No |
| 99–1 | 1 | 1 | No |
| 1–2 c | 1 | 2 | Yes |
| 110–1 | 2 | 2 * | No |
| 46–1 | 2 | 1 | Yes |
| 80–1 | 2 | 2 * | No |
| 127–1 | 2 | 4 | Yes |
| 133–1 | 3 | 3 | No |
| 1–1 | 3 | 2 | Yes |
| 30–4 | 3–4 | 3 | ? |
| 113–12 | 3–4 | 4 | ? |
| 20–2 | 7–10 | 9–10 | ? |
| 197–1 | 7–11 | 8–10 | ? |
| 118–2 | 0 | 5 | Yes |
| Hom.110–1 | 2 § | 2 § | No |
* As no integer in the 95% confidence interval was found, the nearest integer to rline/r1 was chosen. §For the homozigous 110–1 line, containing the double amount of transgene, the copy number was calculated as [(rline/r1)/2].