Table 4.

Comparison between copy number values as estimated by real-time PCR and Southern blotting. The results of estimates of TSWV-N transgene copy number obtained with real-time PCR are compared with those derived from Southern analysis, either limited to one restriction enzyme (KpnI) or extended to several ones.

Line	real-time PCR	Southern (KpnI)	Southern (extended)
111–6	1	1	1
99–1	1	2	1–2
1–2 c	1	1	1–2
110–1	2	1	1–2
46–1	2	1	1–2
80–1	2	1	1–2
127–1	2	4	4–7
133–1	3	2	2–3
1–1	3	n.t.*	n.t.
30–4	3–4	1	1–4
113–12	3–4	2	2–4
20–2	7–10	4	4–8
197–1	7–11	4–6	3–6
118–2	0	0	0
Hom.110–1	2	1	1–2

* not tested