Table 5.
Primers and probes used for quantitative real-time PCR assays. Primers and TaqMan probes were designed on the GenBank sequences indicated. Probes were labelled with FAM and TAMRA at the 5' and 3' ends, respectively.
| apx gene, tomato endogenous (93 bp amplicon) | (from GenBank acc. no. Y16773) |
| Q-TOMAPX-2818(+) | 5'-TTTTACTTGTATATTCGAAGTGTGCCA-3' |
| Q-TOMAPX-2910(-) | 5'-ACAACTGCAAAATTAGAATCTAGTTGGTA-3' |
| Q-TOMATO probe | 5'-TTTCACCTATCTGGACCAAGTTTTGGCAAG-3' |
| TSWV-N transgene (79 bp amplicon) | (from GenBank acc. no. Z36882) |
| Q-TSWVN-492(+) | 5'-TGTCTTGGCTATATATCAGGATGCA-3' |
| Q-TSWVN-570(-) | 5'-TAAGGCTTCCCTGGTGTCATACTT-3' |
| Q-TSWVN probe | 5'-ACAAGGACCTTGGGATCGACCCAAA-3' |
| nptII transgene (60 bp amplicon) | (from GenBank acc. no. U09365) |
| Q-NPT-250(+) | 5'-AGGAAGCGGTCAGCCCAT-3' |
| Q-NPT-309(-) | 5'-GCGTTGGCTACCCGTGATAT-3' |
| Q-NPT probe | 5'-CGCCGCCAAGCTCTTCAGCAA-3' |