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Immunodetection of λ repressor fragments in bacterial fractions LA57 indicates the slow folding λ repressor, LA57-N102LT, while wt refers to the wild type λ repressor, N102LT. The strains used were either wild type secY/prlA or prlA4 as indicated. The individual lanes are as follows: M – purified N102LT used as a molecular weight marker, W – whole cell lysate, S – spheroplast fraction (containing cytoplasmic contents and membranes), P – periplasmic fraction.
