Fig 4.
RecR protein inhibits annealing of plasmid-sized ssDNA by reducing the extent of annealing without affecting the half-time. 3H-ssDNA (3 μM) was incubated with various concentrations of RecR protein for 2 min at 30°C, and then 250 nM RecO protein was added to start annealing. RecR protein concentrations: 0 μM (•), 0.3 μM (▾), 0.6 μM (▴), 0.9 μM (♦), and 1.2 μM (▪). Each data set was fitted by using GRAPHPAD PRISM software with one phase exponential equation: Y = Ymax ⋅(1 − e−kx). There was no significant change in the rate constant k over all RecR protein concentrations.