Fig 5.

UvsY protein has ssDNA annealing activity. Annealing of oligo-25 and oligo-26 (200 nM each) was carried out with UvsY protein (UvsY alone, bold line), gp32 (gp32 alone), or gp32 and UvsY (gp32 → UvsY), or in the absence of protein (spontaneous). The above reactions were started by addition of oligo-26 to the other components that had been preincubated for 100 s in the reaction buffer. Annealing reactions using SSB-coated (SSB → UvsY) or RPA-coated (RPA → UvsY) oligonucleotides were started by the addition of UvsY protein to the oligonucleotides that were preincubated with either SSB or RPA. The final concentrations of gp32, SSB, RPA, and UvsY proteins were 133, 67, 50, and 20 nM, respectively.