Fig 2.

(A) Graphic representation of five apparent KaiA homologs from the listed cyanobacteria. The amino termini are regions of low sequence similarity and are represented by white boxes. The carboxyl termini are regions of high sequence similarity and are represented by black boxes. (B) Graphic representation of the two independent domains derived from S. elongatus KaiA in this study. (C) An SDS/PAGE gel containing samples from a limited trypsin digest assay (see Materials and Methods). As indicated, we observed three major species. Production of the KaiA135N and KaiA180C domains was, in part, based on the largest and smallest of the three species.