Fig 6.

Kinetics of reactivation of luciferase from the GuHCl-denatured state in the absence (○) and the presence of 10 μM DnaK384-638 (•) or 10 μM DnaK386-561 (□). Luciferase denatured in 5 M GuHCl was diluted 100-fold into refolding buffer (100 mM potassium phosphate, pH 7.8/1 mM EDTA/1 mM DTT) at 5°C. The final protein concentration was 61 nM. The activity of native luciferase incubated in refolding buffer containing 50 mM GuHCl is also shown (x).