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Alcian blue and PAS staining of the explants. The acetone-fixed paraffin sections of the explants cultured for 14 days were stained with Alcian blue/PAS solution. (A) Four day-cultured explant. (B) Higher magnification of A. (C) Seven day-cultured explant. (D) Ten day-cultured. (E) Fourteen day-cultured explant. (F) Higher magnification of E. epi, epidermis; mes, mesenchyme; car, cartilage; neu, neural tissue. (Bars = 50 μm.)
