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Expression of X-dll4, Col2, and Cart-1 mRNA in the explants and embryos detected by RT-PCR. RNA was extracted from embryos and explants. ODC was included as an internal RNA loading control. (A) Expression in the explants cultured for 14 days. (B) Expression in the embryos at stage 9, stage 10, and tailbud stages 22–28, 35, and 41.
