Table 1.
Exon 2–5 LWS gene variability in cichlid fishes of Lakes Victoria/Nabugabo
| Species
|
n
|
Heterozygotes
|
Occurrence (frequency) of alleles in species | Undetermined
|
Locality
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 01 | 02 | 03 | 04 | 05 | 06 | 07 | 08 | 09 | 10 | 11 | 12 | 13 | 14 | |||||
| Paralabidochromis beadlei | 3 | 0 | 6 | LN | ||||||||||||||
| P. chilotes | 3 | 1 | 1 | 5 | LV | |||||||||||||
| N. nigrican | 3 | 0 | 4 | 2 | LV | |||||||||||||
| H. nyererei | 3 | 1 | 1 | 5 | LV | |||||||||||||
| Paralabidochromis plagiodon | 3 | 2 | 2 | 1 | 1 | 2 | LV | |||||||||||
| H. pyrrhocephalus | 4 | 0 | 4 | 2 | LV | |||||||||||||
| Gaurochromis simpsoni | 3 | 1 | 2 | 2 | 2 | LN | ||||||||||||
| Astatotilapia velifer | 3 | 0 | 2 | 4 | LN | |||||||||||||
| Haplochromis velvet black | 3 | 3 | 2 | 2 | 2 | LV | ||||||||||||
| Ptyochromis xenognathus | 3 | 0 | 6 | LV | ||||||||||||||
| Ptyochromis sauvagei | 3 | 0 | 6 | LV | ||||||||||||||
| Haplochromis rockkribensis | 3 | 1 | 1 | 5 | LV | |||||||||||||
| Yssichromis laparogramma | 3 | 0 | 2 | 2 | 2 | LV | ||||||||||||
*
Heterozygotes were revealed by the presence of two superimposed nucleotide signals in the sequence profiles obtained by direct sequencing of PCR fragments. “Undetermined” indicates the presence of more than two sites of two superimposed nucleotide signals. Of 264 individuals, 41 tested for exon 4 variability, and 12 of 40 individuals tested for variability in exons 2–5 (16% and 30%, respectively) were heterozygotes. The allelic composition of the heterozygotes could be deduced only when single nucleotide sites varied or when the heterozygosity pattern consisted unambiguously of two alleles present in the same population.
†
LN, Lake Nabugabo; LV, Lake Victoria.