Fig 4.
A screen to identify strains defective in postgermination growth. (A) Homozygous diploid YPD growth (solid lines) and postgermination growth (dotted lines) of deletion strains [hop1Δ/hop1Δ (open circles), mam1Δ/mam1Δ (open squares), arp1Δ/arp1Δ (filled squares), wsc2Δ/wsc2Δ (open triangles), yhl044wΔ/yhl044wΔ (filled circles), and yjl192cΔ/yjl192cΔ (crosses)] with a postgermination growth defect. The curves are plotted as a function of the initial TAG hybridization signal over time. By 15 generations of postgermination growth, all six strains are absent from the pool. (B) A pure spore suspension (≈2 × 107 cells) derived from each diploid deletion strain was germinated in rich media (YPD). We monitored growth by assaying the culture turbidity at 3-hour intervals. Each curve represents the average of three independent experiments. The strain heterozygous for ALG7, an essential gene, is used as a control.