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. 2002 Nov 13;99(24):15542–15547. doi: 10.1073/pnas.242474199

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Copyright © 2002, The National Academy of Sciences

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Fig 3. — Defects associated with the Smad4^m4Mag allele. Hematoxylin and eosin-stained sagittal sections of E7.5 embryos are shown. (A) Wild-type gastrulating embryo showing newly formed mesoderm (arrow) and other well patterned structures. (B) Homozygous Smad4^tm1Cxd embryo. (C) Smad4^m4Mag/Smad4^tm1Cxd embryo. (D) Homozygous Smad4^m4Mag embryo. (B–D) Compared with the wild-type E7.5 embryo (A), these mutant embryos are much smaller, with no sign of the initiation of gastrulation or the formation of mesoderm. Also note the disproportionally large cavity between the visceral and parietal endoderm (asterisks). (E) Steady-state level of SMAD4 protein in unmutagenized wild-type ES cells (lane 1) and three mutagenized sibling subclones (lane 2, wild-type for Smad4; lanes 3 and 4, heterozygous for Smad4^m4Mag). The same blot was stripped and reprobed with an anti-ACTIN antibody to show equal loading of total cellular proteins.