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PTX reduces the efficiency of a single round of R5-enveloped, but not VSV-pseudotyped, HIV-1 virions. HOShigh cells treated with PTX (A) or herbimycin A (B) were exposed to R5-enveloped (▪) or VSV-pseudotyped (•) env-defective HIV-1 R5 virions harboring the luciferase marker gene, and luciferase activity was measured in cell lysates 72 h later. Comparable results were obtained in other experiments (n = 4).
