Table 2.
Sample | Relative expression level | P |
---|---|---|
p53 +/+ untreated | 1.00 ± 0.043 | |
p53 +/+ adriamycin 24 h | 4.315 ± 0.501 | 0.0002 |
p53 +/− untreated | 1.766 ± 0.262 | |
p53 +/− adriamycin 24 h | 2.326 ± 0.418 | 0.0602 |
p53 −/− untreated | 1.181 ± 0.138 | |
p53 −/− adriamycin 24 h | 1.129 ± 0.154 | 0.3452 |
p53 +/+ untreated | 1.05 ± 0.367 | |
p53 +/+ 6 Gy 6 h | 1.059 ± 0.258 | 0.4873 |
p53 +/+ 6 Gy 12 h | 2.313 ± 0.142 | 0.0026 |
p53 +/+ 6 Gy 24 h | 5.044 ± 0.586 | 0.0003 |
Cells were treated with 500 ng/ml adriamycin or γ-irradiation (6 Gy) and mRNA was isolated at the indicated times after treatment.
Values represent the means of two independent experiments in triplicate ± standard deviation, which were standardized by using the 18S rRNA internal control.
P values were calculated by Student's t test comparing treated and untreated samples.