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. 2002 Nov 13;99(24):15655–15660. doi: 10.1073/pnas.232137699

Fig 1.

Fig 1.

Morphology and karyotype of HepaRG cells. Phase contrast micrographs of HepaRG cells under proliferating conditions (A), maintained in culture for 30 days without DMSO (B), and maintained for 15 days without DMSO, then treated with 2% DMSO for 15 days (C). Hepatocyte-like cells and epithelium-like cells are indicated, respectively, by “h” and “e.” A bile canaliculus is indicated by a white triangle. (Bars = 50 μm.) Electron micrographs of HepaRG cells: low magnification view of HepaRG cells (D) and higher magnification views (E and F), showing a typical bile canaliculus-like structure and glycogen accumulation, respectively. (Bars = 2 μm.) RHG banding karyotype of a representative pseudodiploid metaphase of HepaRG cells (G). A karyotype of 46<2n>,XX,+del (7)(q11;q21)inv?(7)(q21q36),-der(22)t(12;22)(p11;q11) was interpreted. The two main anomalies are indicated by arrowheads.