Fig 4.

The C. neoformans MFα1 promoter is specifically expressed in the C. elegans intestine (magnification ×40). Fluorescent microscopy of wild-type C. elegans N2 after feeding for 3 days on C. neoformans H99 expressing GFP fused to the MFα1 promoter. Green fluorescence is seen in yeast cells inside the proximal (A) and the distal (B) end of the C. elegans intestine. There was no fluorescence associated with yeast cells outside the nematodes and no fluorescence was observed on day 1 or 2 of this experiment.