Fig 2.

Detection of multiple viruses by DNA microarray. (A) One small region of a microarray is depicted with its corresponding bar graphic. Each detection oligonucleotide from the microarray is depicted as a vertical stripe. Detection oligonucleotides (stripes) are grouped by viral family of origin, and hybridization intensity on the microarray is reflected by the color of the stripes. Black indicates signal below threshold and Cy5 hybridization intensity above threshold is represented by a continuous color scale (yellow). The upper limit of the linear scale for each microarray was independently set to account for array-to-array hybridization variation. (B) Hybridization results from infection of HeLa cells with adenovirus 12, parainfluenza 3, RSV, polio1, and RV2. Detection oligonucleotides from the herpes and miscellaneous viruses are omitted for clarity (full plots are available in Fig. 5, which is published as supporting information on the PNAS web site, www.pnas.org). (C) Hybridization results from the BCBL-1 cell line, which harbors KSHV. For clarity, only the herpes virus detection oligonucleotides are shown.