Table 3.
Molecular complementation of the cki1 phenotype
| T0 genotype | KanR:KanS | T-DNA transmission, % | No. of complemented plants |
|---|---|---|---|
| cki1-5/CKI1 | 14:9 | 60.9 | 4 (13) |
| cki1-5/CKI1 | 17:7 | 70.8 | 5 (17) |
| cki1-5/CKI1 | 14:9 | 60.9 | ND |
| cki1-5/CKI1 | 19:11 | 63.3 | ND |
| cki1-5/CKI1 | 14:6 | 70.0 | ND |
| cki1-5/CKI1 | 15:6 | 71.4 | ND |
| cki1-5/CKI1 | 21:13 | 61.8 | ND |
| cki1-5/CKI1 | 18:11 | 62.1 | ND |
| cki1-5/CKI1 | 20:11 | 64.5 | ND |
| cki1-5/CKI1 | 20:8 | 71.4 | ND |
| cki1-5/CKI1 | 19:9 | 67.9 | ND |
| cki1-5/CKI1 | 19:8 | 70.4 | ND |
| cki1-5/CKI1 | 24:11 | 68.6 | ND |
| cki1-5/CKI1 | 15:9 | 62.5 | ND |
| cki1-5/CKI1 | 20:11 | 64.5 | ND |
| cki1-5/CKI1 | 19:11 | 63.3 | ND |
| cki1-6/CKI1 | 16:7 | 69.6 | 3 (16) |
| cki1-6/CKI1 | 13:6 | 68.4 | ND |
| cki1-6/CKI1 | 14:7 | 66.7 | ND |
| cki1-6/CKI1 | 16:7 | 69.6 | 5 (15) |
| cki1-6/CKI1 | 20:9 | 69.0 | ND |
| cki1-6/CKI1 | 21:9 | 70.0 | ND |
Each row describes an independent transgenic line. ND, not determined.
*
T0 refers to the plant whose progeny was transformed.
†
Kanamycin segregation data for the T2 generation of transformed plants.
‡
Percent T-DNA transmission was calculated as 100 × KanR/(KanR + KanS).
§
Complemented plants were determined by PCR to have the genotype cki1/cki1 + CKI1 transgene. Numbers in parentheses indicate the total number of KanR plants genotyped.