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. Author manuscript; available in PMC: 2006 Oct 1.
Published in final edited form as: J Immunol. 2005 Oct 1;175(7):4265–4273. doi: 10.4049/jimmunol.175.7.4265

FIGURE 2.

FIGURE 2

DC-SIGN staining is restricted to macrophages in the medullary sinuses (S) of lymph nodes. Two-color staining is shown, with red being the anti-DC-SIGN reagent, and green the other markers. First row, Clone 1 anti-DC-SIGN stains macrophages in the sinuses of the medulla and anti-CD3 stains T cells in the medullary cords (C). Second row, Clone 1 and anti-CD68 colabel medullary macrophages. Third row, Clone 1 and anti-macrophage mannose receptor (MMR) colabel macrophages in the lymph node medulla. Fourth row, Double staining using a commercial anti-DC-SIGN 507 and anti-mannose receptor mAbs.