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. Author manuscript; available in PMC: 2006 Oct 1.
Published in final edited form as: J Immunol. 2005 Oct 1;175(7):4265–4273. doi: 10.4049/jimmunol.175.7.4265

FIGURE 4.

FIGURE 4

Immunostaining of the lymph node cortex with anti-DC-SIGN mAbs. First row, The lymph node cortex is shown at ×10 after staining with anti-CD21 (green) to define the B cell follicles (B) and the unstained T cell region (T). Second row, Images at higher magnification within the T cell region reveal that most DCs defined by staining with anti-CD205 are DC-SIGN negative. Third row, The left panel is a double staining with our clone 10 (specific for DC-SIGN and L-SIGN) and CD11c; the middle panel is a double staining with clone 612 (specific for DC SIGN and L-SIGN) and DEC205; the right panel shows a double staining using anti-L-SIGN specific mAb (604) with MR-1.