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Overview of thioesterase assays. (a) Proline quench assay. (b) Acetyl-S-4′PP-NRPS hydrolysis assay. (c) Acetyl-S-4′PP-ACP hydrolysis assay. (Left) A schematic drawing of the corresponding assays. Compounds shown in red carry a radioactive label. In all cases, a NRPS/thioesterase ratio of 1:0.5 was used.
