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(A) SDS/PAGE mobility of wild-type (wt) and M44K/M64E double-mutant azurin proteins obtained after hyperexpression in E. coli. The cytotoxicity exhibited by wt azurin and M44K/M64E mutant azurin toward UISO-Mel-2 (B) and UISO-Mel-6 (C) cells was determined by the MTT assay as described under Materials and Methods.
