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Calculated radii for the native, intermediate, and unfolded states
| State | Radius, Å |
|---|---|
| Native protein | 16.5 |
| Intermediate state (pH 3) | 15 |
| Intermediate state (pH 2+ 40 mM KCl) | 14.2 |
| Unfolded (at pH 2) | 22 |
Experiments performed in 20 mM phosphate buffer at room temperature.
Experiments performed using 2 mM citrate buffers to adjust the pH.
