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DRM-associated Hxt2 is excluded from the tip of the shmoo. Hxt2-GFP (MBY238) and Hxt2-PA (MBY242) were induced by shifting the cells to low (0.1%) dextrose. Cells were treated with 3 μM α-factor for 3 h and processed as in Fig. 3. Most of Hxt2-PA was found in the DRM fraction (R).
