Fig 5.

Cisplatin resistance and accumulation by mouse embryonic cell lines lacking mCtr1. Cells were incubated for 2 h with cisplatin. For determining survival (A), cells were stained with trypan blue to detect dead cells; unstained cells were counted using a hemocytometer. Data are expressed as percentages of unstained cells compared with control cultures not exposed to cisplatin. For measuring cisplatin accumulation (B), cells were lysed and, after a centrifugation, the supernatant was used to determine platinum content. The platinum reading was normalized to protein concentration.