Fig 3.
NF-E2-dependent H3-meK4 and H3 and H4 acetylation patterns of the endogenous murine β-globin locus. Cells were incubated for 4 days with 1.5% DMSO. (A) H3-meK4 pattern of the murine β-globin locus in DMSO-induced MEL, CB3, CB3-6, and CB3-9. The relative level of H3-meK4 was determined quantitatively and plotted as a function of the position within the locus. (B and C) H3 and H4 acetylation patterns of the β-globin locus in DMSO-induced MEL, CB3, CB3-6, and CB3-9 cells. The relative levels of H3 and H4 acetylation were determined quantitatively and plotted as a function of the position within the locus. Number of independent ChIP samples analyzed: MEL, n = 5; CB3, n = 5; CB3-6, n = 3; and CB3-9, n = 2 (IVR-3, IVR-5, βmajor promoter, IVR-6, and IVR-16); and n = 3 (βmajor 3′ and βminor promoter).