Fig 4.

In contrast to NF-E2, GATA-1 does not induce H3-meK4 at the βmajor promoter. G1E-GATA-1 and G1E cells were incubated for 48 h with 1 μM tamoxifen. (A) Western blot analysis of GATA-1 and ER-GATA-1 expression in total cell lysates from MEL, G1E, and G1E-ER-GATA cell lines. (B) Real-time RT-PCR analysis of β-globin RNA expression in G1E and G1E-ER-GATA cells. Relative expression of β-globin RNA levels normalized to GAPDH. The graph depicts data from four (G1E) and five (G1E-ER-GATA-1) independent RNA isolations. (C) Real-time PCR ChIP analysis of H3 acetylation, H3-meK4, and pol II binding at the βmajor promoter. Samples from three independent ChIP experiments were analyzed quantitatively.