Fig 5.

H3-meK4 at hypersensitive site 2 is NF-E2- and GATA-1-independent. MEL and CB3 cells were incubated for 4 days with 1.5% DMSO. G1E-GATA-1 and G1E cells were incubated for 48 h with 1 μM tamoxifen. Quantitative PCR ChIP analysis of H3-meK4 at HS2 in MEL, CB3, G1E-ER-GATA-1, and G1E cells was done. Samples from five (MEL and CB3) and three (G1E-ER-GATA-1 and G1E) independent ChIP experiments were analyzed quantitatively, relative to a standard curve generated from input chromatin. The plot depicts relative levels of H3-meK4 at HS2 in the cell lines.