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. 2002 Oct 14;99(22):14392–14397. doi: 10.1073/pnas.212410199

Fig 6.

Fig 6.

BCR stimulation induces higher AKT Ser-473 and S6 ribosomal protein phosphorylation in hTCL1 vs. wild-type spleen cells. Six- to 8-week-old hTCL1 (▪) or wild-type (♦) spleen cells were stimulated with 20 μg/ml anti-IgM F(ab′)2 for 2 (or 3), 10, and 60 min, harvested, and immunoblotted. (A Upper) Three representative Western blots for AKT Ser-473 phosphorylation (pAKT), total AKT, and TCL1 from one experiment. (Lower) Densitometry results of three independent stimulation experiments in which the line graphs represent the ratio of Ser-473-phosphorylated AKT to total AKT measured. (B Upper) Immunoblots of Ser-235/236 S6 ribosomal protein phosphorylation (pS6), an actin loading control, phospho-Thr-202/Tyr-204 ERK1 and ERK2 (pERK1 and pERK2) and total ERK1 and ERK2 from a single stimulation experiment. (Lower) Densitometry results for pS6 to actin (iv and v) and pERK1 and pERK2 to total ERK1 and ERK2 ratios (vi and vii) for two independent stimulation experiments.