Fig 3.
Expression analyses of CSF-1R in human prostate cancer cell lines. (A) RT-PCR analysis. The PCR products at representative cycles were shown: CSF-1R, CSF-1, and prostate-specific antigen, cycle 40 and β-actin, cycle 25. The primers sequences are listed in Materials and Methods. (B) Immunoblotting analysis. Protein lysates of each 2 × 105 cells were subjected to SDS gel electrophoresis and polyclonal rabbit anti-human CSF-1R antibodies (1:250 dilution) were used for this analysis, as described in Materials and Methods. Human breast cancer cell line BT-20; human prostate cancer cell lines PC3, DU-145, LNCap, and LAPC4, and normal basaloid PrEC prostate cells were used in these analyses. PC-3 infected with human CSF-1R expressing retrovirus was used as a control.