Fig 2.

Intrahepatic inoculation of chimpanzee 1580 with RNA transcripts of the wild-type Con1 clone (pFK-Con1). Serum samples were tested for HCV-RNA by in-house RT-nested PCR with 5′ UTR primers and/or the Roche Monitor Test 2.0: filled rectangle, positive by RT-nested PCR and/or by Monitor; open rectangle, negative by RT-nested PCR in two independent assays; open circle, HCV Monitor titers; samples found to be below the detection limit of 600 units/ml (indicated by dashed line) are shown as not detected (ND). Seroconversion in the Abbott second-generation ELISA for anti-HCV is represented by a horizontal bar. Shaded area, serum levels of ALT. Liver Histology: necroinflammatory changes of liver biopsy samples graded as 0 (normal), 1 (mild), 2 (mild–moderate), 3 (moderate–severe), or 4 (severe).