Table 1.
Evolution of HCV-Con1 in chimpanzee 1580
| Week p.i.
|
E1 347 M
|
E2 430 N
|
p7 769 L
|
NS2 1017 S
|
NS3 | NS5A | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1177 V | 1444 F | 1498 I | 1568 I | 2356 E | 2362 S | 2375 D | |||||
| 1 | M | N | L | S | V | F | I | I | E | S | D |
| 2 | |||||||||||
| 3 | S | ||||||||||
| 4 | S | ||||||||||
| 5 | S | ||||||||||
| 6 | S | ||||||||||
| 6.5 | S/n | ||||||||||
| 7 | S/n | ||||||||||
| 8 | S/N | ||||||||||
| 8.5 | M | N | L | N/s | V | F | I | I | E | S | D |
| 28 | I/m | N/D | I/l | D | V | Y | I/V | I/M | E/g | N | D/g |
| 40 | I | D | I | D | A | Y | V | M | G | N | G |
| 52 | I | D | I | D | A | Y | V | M | G | N | G |
The consensus sequence of the entire ORF of viruses recovered from chimpanzee 1580 at weeks 1, 8.5, 28, 40, and 52 after transfection was determined. Only amino acid changes are given. The corresponding amino acids and their positions in the Con1 polyprotein sequence are shown on top. The differences from the wild-type Con1 sequence are indicated below. Dominant sequences are shown in capital letters; minor sequences are shown in lowercase letters. For sequential analysis of the NS2 position with a substitution identified at week 8.5, the consensus sequence of only a short genomic region was determined during weeks 3–8.
*
Entire polyprotein analyzed.