Fig 3.

APP processing by wild-type, PS1^−/− Notch-mild and Notch-severe mice. (a) Western blot of wild-type, Notch-severe, and Notch-mild B6 × 129 F₂ PS1^−/− brain protein lysates incubated with an APP-specific antibody. The result shows equal amounts of full-length APP (FL-APP) among all three brains. Although no APP C-terminal fragment (CTF-APP) was observed in the wild-type brains, it was equally evident among all Notch-severe and Notch-mild brains. (b) Results of ELISAs for Aβ₄₀ and Aβ₄₂ from PS1^+/+, PS1^+/−, and Notch-severe and Notch-mild PS1^−/− brains.