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SR2-GFP recycles back to the surface after ligand-independent PKC-mediated internalization. (A) Control before application of PMA. SR2-GFP internalized in 10 min after exposure to 100 nM PMA (B). Cells were then washed free of PMA and incubated at 37°C for 1 h (C), 1.5 h (D), 2 h (E), and 2.5 h (F). The receptor recycled back to the surface after 2.5 h of incubation in the absence of PMA (F).
