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. 2002 Oct 21;99(22):14500–14505. doi: 10.1073/pnas.222371099

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Copyright © 2002, The National Academy of Sciences

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Fig 1. — Construction and histological characterization of ^GFPKIF17 transgenic mice. (A) The construct for production of ^GFPKIF17 transgenic mice. (B) PCR screening with GFP primers, using endogenous MPG as internal control. (C) Visualization of motility of ^GFPKIF17. (Bar = 20 μm.) (D) Immunoprecipitation of the forebrain fraction from mouse using anti-GFP antibody; the same immunoblot was used for antibodies against KIF17, Mint 1(mLin10), NR2B, and IgG. (E) Normal brain morphology in transgenic mice compared with WT, hematoxylin/eosin staining (Top), higher magnification of the hematoxylin/eosin staining (Middle), and Nissl staining (Bottom) of the hippocampus. (Bars = 500 μm.) (F) The ultrastructure of axospinous synapses in CA1 hippocampus shown in each case. (Bar = 10 μm.) (G) ^GFPKIF17 localization in forebrain region of mice. Neocortex and hippocampal subregions (CA1, CA3, DG). (Bar = 100 μm.)