Skip to main content
. 2002 Oct 16;99(22):14578–14583. doi: 10.1073/pnas.212522099

Fig 4.

Fig 4.

Expression of alfalfa CHI in Arabidopsis. (A) Binary alfalfa CHI construct. B, BamHI; S, SalI; T, rubisco 3′ terminator. (B) Complementation of the tt5 mutation by transformation with alfalfa CHI. (C) RNA gel blot analysis of alfalfa CHI transcripts in independent transformants in WT (Col-0) and pap1-D backgrounds. (D) Transgene CHI activities in independent transformants in Col-0 or Col-0/pap1-D backgrounds. (E and F) HPLC profiles of nonhydrolyzed extracts from leaves of an empty vector plant (E) and from lines 4–11 expressing alfalfa CHI (F). (G and H) HPLC profiles of β-glucosidase-treated extracts from leaves of an empty vector plant (G) and from lines 4–11 (H). Compounds are: a, Rha-Gluc-Rha-quercetin; b, Rha-Gluc-Rha-kaempferol; c, Rha-Rha-Q; d, Rha-Rha-K; e, Sinapic acid; f, Gluc-Rha-Q; g, rutin; l, kaempferol, p, quercetin; k and n, unidentified kaempferol conjugates; o, unidentified quercetin conjugate; h, i, j, and m, other unknown flavonol conjugates.