Table 2.
Genistein, flavonol, and anthocyanin levels in transgenic Arabidopsis expressing soybean IFS, alfalfa CHI, and over-expressing PAP1
| Line | Generation | Quercetin, nmol/g FW | Kaempferol, nmol/g FW | Total flavonols, nmol/g FW | Anthocyanins, Au/g FW | Genistein, nmol/g FW |
|---|---|---|---|---|---|---|
| Wild-type | >T3 | 21.0 ± 5.7 | 418.9 ± 33.3 | 439.9 ± 30.9‡ | 3.1 ± 0.4‡ | 0‡ |
| Pap1-D | >T3 | 200 ± 8.3 | 345.5 ± 48.3 | 545.7 ± 40.3‡§ | 192.0 ± 7.1§ | 0‡ |
| CHI/Pap1-D (3–26) | >T3 | 89.3 ± 6.1 | 666.6 ± 103.0 | 755.9 ± 103.7§∥ | 131.1 ± 10.6¶ | 0‡ |
| IFS 6E | >T3 | 20.8 ± 6.6 | 323.5 ± 4.3 | 344.3 ± 9.5¶ | 1.1 ± 0.2∥ | 3.5 ± 0.2§ |
| 3–26/6E | F2 | 72.8 ± 12.1 | 1029.8 ± 84.6 | 1102.6 ± 91.1∥ | 30.7 ± 5.7 | 4.8 ± 2.4§ |
*
Plants were grown under 12 h light/12 h dark, ∼200 μE light intensity. Data for >T3 plants are means ± SE from three replicate plants with three independent analyses for each plant. Data for F2 progeny represent triplicate analyses of five plants (see supporting information).
†
UV absorbance at 510 nm.
‡§¶∥
**
Symbols represent pairwise statistical analyses. Different symbols represent significantly different values at P ≤ 0.05; numbers with the same symbols are not significantly different at P > 0.05.