Table 1.
Effect of persistent organochlorines on transient BRCA1-luciferase (pGL12) reporter gene expression in MCF-7(BUS) and MDA-MB-231
| MCF-7(BUS) | Tox.Tech. | Tox.Tech. +100 pM E2 | ICI 182780 | ICI 182780 +100 pM E2 | TCDD | TCDD +100 pM E2 |
| Control | 18 ± 2.9 | 100 ± 11.8 | 18 ± 0.3 | 100 ± 6.3 | 18 ± 0.8 | 100 ± 8.4 |
| 10 μM | 36 ± 8.3* | 59 ± 8.6* | 5 ± 1.1* | 8 ± 3.4* | n.d. | n.d. |
| 1 μM | 20 ± 2.5 | 96 ± 25.2 | 7 ± 0.5* | 7 ± 0.6* | n.d. | n.d. |
| 100 nM | 20 ± 6.8 | 102 ± 16.8 | 5 ± 0.4* | 5 ± 1.7* | 7 ± 0.9* | 46 ± 4.6* |
| 10 nM | 24 ± 8.1 | 95 ± 7.4 | 5 ± 0.7* | 8 ± 2.4* | 7 ± 0.8* | 33 ± 2.6* |
| 1 nM | 23 ± 8.1 | 88 ± 21.8 | 5 ± 0.7* | 46 ± 10.8* | 8 ± 1.3* | 39 ± 3.5* |
| 100 pM | 24 ± 9.3 | 108 ± 11.4 | 9 ± 0.3* | 81 ± 5.0* | 14 ± 2.5* | 66 ± 12.6* |
| 10 pM | 17 ± 1.7 | 112 ± 4.2 | 12 ± 0.8* | 109 ± 25.1 | 16 ± 4.0 | 110 ± 24.1 |
| 1 pM | 21 ± 3.4 | 92 ± 18.9 | 15 ± 2.3 | 101 ± 55.8 | 25 ± 6.7 | 107 ± 13.8 |
| MCF-7(BUS) | PCB#138 | PCB#138 +100 pM E2 | PCB#153 | PCB#153 +100 pM E2 | PCB#180 | PCB#180 +100 pM E2 |
| Control | 18 ± 3.0 | 100 ± 7.8 | 18 ± 2.1 | 100 ± 6.9 | 18 ± 1.7 | 100 ± 12.7 |
| 10 μM | 7 ± 1.2* | 59 ± 14.9* | 8 ± 0.7* | 75 ± 9.0* | 9 ± 3.5* | 70 ± 11.5* |
| 1 μM | 17 ± 1.5 | 81 ± 19.5 | 16 ± 3.5 | 92 ± 17.2 | 13 ± 4.0 | 105 ± 11.7 |
| 100 nM | 16 ± 1.7 | 90 ± 4.9* | 21 ± 0.9 | 97 ± 12.9 | 16 ± 1.4 | 110 ± 24.7 |
| 10 nM | 17 ± 3.9 | 82 ± 10.5* | 22 ± 4.4 | 113 ± 10.6 | 19 ± 5.4 | 107 ± 28.9 |
| 1 nM | 19 ± 3.0 | 87 ± 4.0* | 20 ± 5.1 | 131 ± 28.9 | 23 ± 7.9 | 94 ± 10.2 |
| 100 pM | 18 ± 4.1 | 88 ± 6.5* | 21 ± 6.4 | 95 ± 16.5 | 17 ± 6.4 | 108 ± 31.1 |
| 10 pM | 19 ± 2.2 | 85 ± 21.2 | 22 ± 0.9 | 112 ± 26.7 | 21 ± 7.4 | 112 ± 11.3 |
| 1 pM | 24 ± 5.3 | 86 ± 20.3 | 19 ± 6.2 | 111 ± 9.5 | 16 ± 5.0 | 109 ± 21.2 |
| MDA-MB-231 | Control | Tox.Tech. (10 μM) | TCDD(10 nM) | PCB#138 (10 μM) | PCB#153 (10 μM) | PCB#180 (10 μM) |
| pGL12 | 93 ± 19.3 | 21 ± 8.1* | 65 ± 11.5* | 35 ± 1.3* | 50 ± 10.6* | 49 ± 7.0* |
| pGL12 + 10 nM E2 | 100 ± 15.0 | 21 ± 6.6* | 69 ± 8.6* | 32 ± 6.9* | 53 ± 6.0* | 44 ± 5.6* |
| pGL12/ER | 57 ± 10.4 | 35 ± 11.6* | 55 ± 15.5 | 45 ± 10.6 | 46 ± 12.8 | 48 ± 13.5 |
| pGL12/ER + 10 nM E2 | 100 ± 13.8 | 37 ± 21.3* | 74 ± 35.8 | 65 ± 23.9* | 60 ± 24.5* | 58 ± 36.5 |
All values are corrected with β-galactosidase values (MCF-7(BUS)) or protein (MDA-MB-231) and then normalized to 17β-estradiol (E2)-induced cells (set to 100). The data are mean ± standard deviations of at least five or six measurements from two independent experiments. pGL12/ER, cotransfection with the pHEO1-expressing estrogen receptor alpha; Tox.Tech., toxaphene (technical mixture); TCDD, 2,3,7,8 tetrachlorodibenzo-p-dioxin; PCB, polychlorinated biphenyl. *P < 0.05 relative to the given control (ethanol/DMSO or E2).