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A single nucleotide substitution in DNA-PKcs in XR-C2 introduces a G→E interchange six amino acids from the C-terminus. A 1.3 kb RT–PCR product from XR-C2 was cloned and sequenced. A single substitution was defined and compared to DNA-PKcs sequenced from the parental cell line CHO9. The substitution results in a glycine to glutamic acid substitution at six amino acids from the C-terminus as indicated.
