Table 1. G4122E mutant DNA-PKcs supports reduced levels of VDJ recombination.

Expression vectors	Experiment	Signal (pJH201) AmpR + CamR / AmpR	Ratea (%)	Fidelityb (%)	Coding (pJH290) AmpR + CamR / AmpR	Ratea (%)
No RAGS	1	0/5560	0.00		0/22 380	0.00
	2	0/660	0.00		0/0	0.00
	3	0/91 080	0.00		1/114 960	0.00
	4	0/85 840	0.00		1/40 368	0.00
RAGS	1	11/6380	0.17		0/44 000	0.00
	2	0/840	0.00		0/0	0.00
	3	31/63 120	0.05		0/53 760	0.00
	4	40/22 736	0.18		0/79 576	0.00
			0.09 ± 0.04c
Wild-type DNA-PKcs	1	127/3300	3.85	70 (7/10)	114/21 000	0.54
	2	7/720	0.90	ND	10/3120	0.32
	3	496/32 520	1.52	80 (8/10)	402/60 360	0.67
	4	230/9976	2.31	100 (7/7)	345/49 300	0.70
			2.2 ± 0.64c	86.7 ± 7.6d		0.56 ± 0.09c
G4122E DNA-PKcs	1	34/3760	0.90	90 (9/10)	8/16 080	0.05
	2	2/1560	0.13	ND	4/2640	0.16
	3	140/31 080	0.45	89 (8/9)	50/100 800	0.05
	4	188/35 380	0.53	90 (9/10)	54/57 072	0.09
			0.50 ± 0.16c	89.0 ± 0.3d		0.09 ± 0.03c

^aRate is the percentage of replicated substrates that underwent V(D)J recombination and is derived from the formula [Amp^R + Cam^R / Amp^R] × 100.

^bFidelity is expressed as the percentage of recovered signal joints that were susceptible to ApaLI digestion.

^cAverage recombination rate ± SEM.

^dAverage fidelity ± SEM.