Figure 3.

Effects of Caudal on luciferase expression driven by the dE2F promoter. Transfections were performed with Drosophila S2 cells and promoter activities measured as luciferase activity normalized to β-galactosidase activity at 48 h thereafter. (A) Each transfection mixture contained the E2F-Luc reporter plasmid, the pAc-lacZ internal control plasmid and the indicated amount of Caudal expressing plasmid, pAc-cad. (B) Constructs carrying a point mutation in Caudal binding sites in the dE2F promoter were cotransfected with the indicated amount of pAc-cad. The mean activities ±SE from three or four independent transfections are shown.