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Expression of caudal and dE2F in hs-GAL4/UAS-cad third instar larvae. Total RNA was prepared from the third instar larvae carrying hs-GAL4 and UAS-cad after heat shock at 37°C for 30 min and incubated at 25°C for various time periods. Then, RT–PCR was performed to measure caudal and dE2F mRNA levels. The line carrying UAS-cad was used as a control and processed in the same way as that carrying hs-GAL4/UAS-cad.
